A culture-dependent survey of thermophilic bacteria from hot springs in Xiamen area in China     

YANG Bo  OUYANG Jianping  AO Jingqun  CHEN Xinhua 《海洋学报(英文版)》2009,28(1):62-71

Microbes are believed to play important roles in ecosystem function in many environments. The hot springs of Xiamen Island are close to the Xiamen Sea, and may have some characteristics different from those of inland hot springs. Microbes living in the hot springs of Xiamen may have new characteristics. However, little is known about microbial communities of hot springs close to the Xiamen Sea. A culturedependent survey of microbial population in the Xiamen hot springs was performed by using an approach combining total cellular protein profile identification and 16S rRNA gene sequencing. A total of 328 isolates of bacteria were obtained from liquid and sediment samples from the Xiamen hot springs, including neutrophilic thermophilic bacteria and moderately thermophilic acidophiles. Neutrophilic thermophilic bacteria, which grow at a temperature range of 55—90℃ including Rhodothermus marinus (Strain 1), Thermus thermophilus (Strain 2), Thermus thiopara (Strain 3), Geobacillus stearothermophilus (Strain 4), Geobacillus thermoleovorans (Strain 5), and Pseudomonas pseudoalcaligenes (Strain 6), were recovered by 2216E plates. Moderately thermophilic acidophiles, which can grow at temperatures above 50℃ and a pH range of 1.8—3.5 such as Alicyclobacillus acidoterrestris (Strain 8), Sulfobacillus acidophilus (Strain 9), and Sulfobacillus thermosulfidooxidans (Strain 10), were isolated on selective solid medium containing sulfur and Fe2+. Among these strains, Rhodothermus marinus, Thermus thermophilus and Geobacillus stearothermophilus are not only thermophiles, but also halophiles. One bacterium strain (Strain 6) shared 99% nucleotide sequence homology with Pseudomonas pseudoalcaligenes on the 16S rRNA gene sequence, but was quite different from Pseudomonas pseudoalcaligenes in biological characteristics, suggesting that it may represent a novel thermophilic species. Results indicated that various species of neutrophilic thermophiles and moderately thermophilic acidophiles were widely distributed in the Xiamen hot springs and that Rhodothermus marinus and Thermus thermophilus dominated the cultivable microbial community.  相似文献   

语义Web本体语言及OWL研究     

张蕾 《成都信息工程学院学报》2007,22(2):161-165

面对Internet应用模式的变化和Web海量信息的冲击,Web信息个性化、智能化的研究不断深入.语义Web规划对当前的万维网进行扩展,期望用加入信息中的语义内容导航信息资源检索并提高网络服务的智能化,使机器自动与人协同工作.目前,虽然语义Web还是个理想模型,但将语义Web融入现有Web结构进行信息智能开发的初步努力已经展开.特别是随着对本体技术研究的日渐深入,OWL本体理论探索和应用开发逐渐受到广泛重视.  相似文献   

4种酞酸酯在龙须菜-篮子鱼食物链中的积累放大研究   总被引：1，自引：0，他引：1       下载免费PDF全文

聂湘平  李桂英  吴志辉  李潇  杨宇峰 《海洋科学》2008,32(1):19-23

在模拟受控条件下,利用气相色谱方法研究分析了4种酞酸酯类化合物在龙须菜(Gracilaria lemane-iformis)-篮子鱼(Siganus oramin)食物链中的积累、分布和放大状况。结果表明,4种酞酸酯在龙须菜组织内的积累表现为:邻苯二甲酸二异辛酯(DEHP)>邻苯二甲酸二丁酯(DBP)>邻苯二甲酸二乙酯(DEP)>邻苯二甲酸二甲酯(DMP)。侧链最长的DEHP在龙须菜藻体内的含量随暴露时间的延长呈增加的趋势,30 d体内积累量可达2.35 mg/kg(鲜质量);侧链最短的DMP在龙须菜藻体内的质量比随时间变化差异不显著,约为0.05 mg/kg;在30 d的暴露中,4种酞酸酯DMP,DEP,DBP和DEHP在篮子鱼体内总质量比分别为0.08,0.41,1.72,3.11 mg/kg。其中DBP和DEHP在鱼内脏组织中有较高的积累和分布,分别为1.14 mg/kg和1.83 mg/kg,其次为鱼残体组织,肌肉组织中含量最低。DEHP和DBP在龙须菜和篮子鱼体不同组织中均有一定程度的富集(生物富集系数FBC均大于1)。其中DEHP和DBP在内脏组织中的FBC值分别高达9.16和5.68,在龙须菜-篮子鱼这个简单食物链传递中DEHP和DBP在篮子鱼内脏组织中存在放大现象。  相似文献   

Optimum temperature for the growth performance of juvenile orange-spotted grouper （Epinephelus coioides H.）     

林祥志  解绶启  苏永全  崔奕波 《中国海洋湖沼学报》2008,26(1):69-75

Effects of water temperature （17, 21, 25, 30 and 35℃） and body size （14.75-281.41 g initial body weight） on food consumption, growth, feed conversion, and dry matter content in orange-spotted grouper fed to satiation were investigated. The combined effect of temperature （T, ℃） and body weight （W, g） on maximum food consumption （Cmax, g/day） was described as： InCmax=-7.411＋0.828 InW＋0.317T4）.004 7T2, and the optimum feeding temperature was 33.9℃. The combined effect of temperature and body weight on growth （G） was described as： lnG=-4.461-0.2081nW＋0.394T-0.006 3T^2. The optimum growth temperature was 31.4℃, whereas overall growth rates were high at 25, 30 and 35 ℃. Feed conversion efficiencies （FCE, %）, increasing first and then decreasing with increasing temperature, averaged from 1.8 to 2.1 in terms of dry weight of food fish. The optimum temperature for FCE tended to be lower than that for growth or feeding. Dry matter content increased with both increasing water temperature （17, 25, 30 and 35℃） and body weight, and the combined effect of temperature and body weight on dry matter content （DM, %） was described as： lnDM =3.232＋0.01 4 lnW-0.004 4T＋0.001 2TInW.  相似文献   

旅游地传统饮食文化原真性感知对满意度与行为意向的影响——以宏村臭鳜鱼为例     

莫宏伟  尹寿兵  刘云霞 《热带地理》2022,42(11):1918-1930

在区分地方饮食与传统饮食概念的基础上,选择宏村传统饮食臭鳜鱼作为研究对象,通过对就餐环境、服务、食物3个原真性维度和满意度与行为意向2个维度,构建Amos结构方程模型,探究游客对传统饮食文化的原真性感知及其对旅游地就餐满意度和行为意向的影响。结果显示,3个维度的原真性均对满意度呈显著、直接、正向影响,对行为意向呈显著、间接、正向影响;其中,环境原真性对就餐满意度影响最大,其次为服务原真性,最后为食物原真性。研究认为,即使体验对象是与原真性关联紧密的传统饮食,大众游客仍更关注整体就餐环境和服务氛围,食物本身对满意度的影响最低。游客需求引发传统饮食文化舞台化重塑,并呈现“去传统化”与“去地方化”特征,旅游地饮食文化塑造对环境的关注大于食物,但当地居民更加关注食物本身的口味,主客关注差异和食物生产过程的游客需求导向引发当地居民对舞台化饮食的认同度降低。未来旅游地要充分考虑传统饮食的特殊性,重塑让游客与本地居民更加满意的饮食文化。  相似文献   

1637—1643年华北地区极端干旱事件中的粮食危机     

孟佳颖 《地域研究与开发》2022,41(1):169-174

粮食作为自然生态系统与社会经济系统的关联点,能够有效地揭示极端干旱事件对自然-经济-社会复合生态系统影响的过程和机理。以粮价异动为切入点,通过粮价通胀指数和“无透雨”的持续时间,分析农业旱情及其对粮食生产的影响,对1637—1643年华北地区极端干旱事件中的粮食安全进行深入剖析。结果显示:华北地区无透雨天数超过150 d,年降水量与5—9月降水量的距平值均≤-30%,水分异常短缺,土壤严重失墒,危及粮食生产安全。因此,降水量距平值≤-30%是华北地区粮食生产安全的临界阈值。此次极端干旱事件是历史同类事件之最,连续无透雨日数最长,地表径流枯竭记录最多,水资源的短缺导致伴生灾害相继发生,超过生态系统的忍耐极限,粮食生长期缩短,减产歉收,绝产绝收,粮食供给链条断裂。粮价通胀指数最低9.50,最高26.70;货币发行与粮食供给失衡,通货膨胀严重,粮价飞涨,市场倒闭,粮食安全体系崩塌,自然-经济-社会复合生态系统发生质变成为历史必然。  相似文献   

短期增温和降水减少对沙质草地土壤微生物量碳氮和酶活性的影响     

王怀海  黄文达  何远政  牛亚毅  朱远忠 《中国沙漠》2022,42(3):274-281

通过模拟气候变化,探究短期增温和降水减少对沙质草地土壤微生物量碳氮及酶活性的影响,揭示沙质草地土壤微生物量碳氮和酶活性对短期气候变化的响应规律。结果表明：（1）短期增温和降水减少对土壤微生物量碳氮和酶活性均产生显著影响。（2）在自然温度下,与自然降水相比,降水减少40%时土壤微生物量碳（MBC）和微生物氮（MBN）含量最高,增幅分别为87.9%和98.8%;降水减少60%时土壤碱性蛋白酶（S-ALPT）活性最低,降幅达32.8%。（3）在增温条件下,与自然降水相比,降水减少40%时土壤MBC和MBN含量最低,降幅分别为25.67%和48.16%,土壤脲酶（S-UE）活性最高,增幅20.42%。（4）土壤pH与3种土壤酶活性正相关,与土壤微生物量碳氮负相关。土壤微生物量碳氮与土壤纤维素酶（S-CL）活性负相关,与S-UE、S-ALPT活性正相关。  相似文献   

Analysis of Food Consumption and Its Characteristics in Uzbekistan based on the Emergy Method     

JIA Mengmeng  ZHEN Lin  ZHANG Changshun 《资源与生态学报(英文版)》2022,13(5):842-850

Food resources play a great role in human livelihood, so it is meaningful to investigate their utilization and structural evolution, especially in developing countries. This study takes data from FAOSTAT and the World Bank, and applies the emergy method to analyze Uzbek food consumption changes and characteristics from 1992 to 2019. Two main results were obtained: (1) National food consumption showed a persistent increase and had a remarkable change in stages, with the first stage of low-level repetition, the second stage of speeding up and the third stage of high development. The increase of animal-based food consumption was more notable than that of plant-based food consumption. (2) Per capita annual food consumption had a tendency of change that was similar to that of national food consumption. The proportion of animal-based food in the total per capita annual food consumption usually remained about 2-fold higher than that of plant-based food consumption. The main food consumption pattern was the combination of crops such as wheat and rice with meat such as beef. The fruit and vegetable consumption also increased. With the growing population, various changes in food demand will inevitably happen, so it is necessary to take sustainable measures in time to satisfy the new demand and to protect resources and the environment.  相似文献   

基于Web数据挖掘模型的研究     

袁勇  万幼川 《地理空间信息》2004,2(3):22-24

阐述了Web数据挖掘的概念和特征,在分析XML技术的基础上,介绍了基于XML的Web数据挖掘模型,并对Web数据挖掘的应用领域和范围,尤其是在电子商务方面的应用进行了探讨。  相似文献   

多源道路智能选取的本体知识推理方法EI北大核心CSCD     

郭漩  钱海忠  王骁  刘俊楠  任琰  赵钰哲  陈国庆 《测绘学报》2022,51(2):279-289

大数据时代道路数据来源日益增多,跨数据源的道路选取面临巨大挑战。本文针对数据语义不一致问题,提出一种基于本体知识推理的多源道路选取方法。首先,将1∶5万基本比例尺地形图道路数据作为基础案例,将四维图新导航电子地图和开放街道地图中的道路数据作为试验数据,基于stroke计算道路等级、长度、连通度、接近度、中介度特征项,提取特征项概念并构建本体;然后,从语义特征项和数值特征项两方面计算本体概念相似性,建立基础案例与试验数据间的关联关系;最后,基于本体和语义网规则语言定义本体通用、语义特征、数值特征三类选取规则,实现跨数据源道路选取的过程性知识推理。试验表明,本文方法可基于本体概念相似性度量消除语义差异,同时利用语义网规则语言进行知识推理,可实现多源道路数据向基本比例尺数据的智能选取。  相似文献